Data Sheet: Sequencing

RNA sequencing (RNA-Seq) is a powerful method for discovering, annotating, and quantifying RNA transcripts that is currently revolutionizing the field of agrigenomics.1–9 RNA-Seq does not require speciesor transcript-specific probes, enabling precise quantification of both known and novel transcripts without prior knowledge. Beyond the measurement of gene expression changes, RNA-Seq can be used for discovery applications such as identifying alternative splicing events, gene fusions, allele-specific expression, and rare and novel transcripts.